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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
25/10/2017 |
Data da última atualização: |
06/05/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEGORARO, C.; STORCH, T. T.; CRIZEL, G. R.; ROMBALDI, C. V.; GIRARDI, C. L. |
Afiliação: |
Camila Pegoraro, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Tatiane Tim Storch, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Gisele Rodrigues Crizel, Brazilian Agricultural Research Corporation, Embrapa Uva e Vinho, Bento Gonçalves, Brazil; Cesar Valmor Rombaldi, Federal University of Pelotas, Pelotas, Brazil; CESAR LUIS GIRARDI, CNPUV. |
Título: |
Identification of biomarkers associated to 'Gala' apples ripening and postharvest quality . |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Comunicata Scientiae, v. 7, n. 4, p. 494-503, 2016. |
DOI: |
10.14295/CS.v7i4.1545 |
Idioma: |
Inglês |
Conteúdo: |
Apple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period. Keywords: Cold storage, gene expression, Malus x domestica, molecular analysis |
Palavras-Chave: |
Amadureceimento; Análises moleculares; Armazenamento refrigerado; Biomarcadores; Expressão genética; Maçã gala; Molecular analysis. |
Thesagro: |
Maçã; Malus doméstica; Pós-Colheita. |
Thesaurus Nal: |
cold storage; gene expression. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165545/1/artigo-biomarcadores.pdf
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Marc: |
LEADER 02436naa a2200325 a 4500 001 2078129 005 2019-05-06 008 2016 bl uuuu u00u1 u #d 024 7 $a10.14295/CS.v7i4.1545$2DOI 100 1 $aPEGORARO, C. 245 $aIdentification of biomarkers associated to 'Gala' apples ripening and postharvest quality .$h[electronic resource] 260 $c2016 520 $aApple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period. Keywords: Cold storage, gene expression, Malus x domestica, molecular analysis 650 $acold storage 650 $agene expression 650 $aMaçã 650 $aMalus doméstica 650 $aPós-Colheita 653 $aAmadureceimento 653 $aAnálises moleculares 653 $aArmazenamento refrigerado 653 $aBiomarcadores 653 $aExpressão genética 653 $aMaçã gala 653 $aMolecular analysis 700 1 $aSTORCH, T. T. 700 1 $aCRIZEL, G. R. 700 1 $aROMBALDI, C. V. 700 1 $aGIRARDI, C. L. 773 $tComunicata Scientiae$gv. 7, n. 4, p. 494-503, 2016.
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Embrapa Uva e Vinho (CNPUV) |
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